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Recombinant HDAC6 protein
| Aliases: HDAC-6, Histone Deacetylase 6, HD6 |
| 商品コード: 31543 | Format: 20 µg | ¥96,000 | Buy |
| 商品コード: 31943 | Format: 1 mg | ¥788,500 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Protein Details
Recombinant human HDAC6 was expressed in a baculovirus expression system as the full length protein (accession number NP_001308154.1) with a C-terminal FLAG-Tag. The molecular weight of the protein is 135.7 kDa. The recombinant protein is >85% pure by SDS-PAGE.
Recombinant HDAC6 protein
10% SDS-PAGE Coomassie staining
MW: 133.8 kDa
Purity: ≥ 85%
HTRF assay for Recombinant HDAC6 protein
1 μM H3K9ac (1-21 aa) peptide was incubated with different concentrations of HDAC6 protein in 10 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 30 min, then 10 μl anti-H3K9me0 antibody and SA XL665 mixture (1:100 dilution in HTRF. Detection Buffer) was added to each reaction system and incubated for 30 min. All the reactions and operations were performed at room temperature.
HTRF assay for Recombinant HDAC6 protein
1 μM H3K9ac (1-21 aa) peptide was incubated with different concentrations of HDAC6 protein in 10 μl reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 30 min, then 10 μl anti-H3K9me0 antibody and SA XL665 mixture (1:100 dilution in HTRF. Detection Buffer) was added to each reaction system and incubated for 30 min. All the reactions and operations were performed at room temperature.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
