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Recombinant HDAC6 (H230A) protein
| Aliases: HD6, JM21, CPBHM, PPP1R90 |
| 商品コード: 31564 | Format: 20 µg | ¥80,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Protein Details
Recombinant human HDAC6 (H230A) was expressed in a baculovirus expression system as the full length protein (accession number NP_001308154.1) with a point mutation His230Ala and a C-terminal FLAG-Tag. The molecular weight of the protein is 135.7 kDa. The recombinant protein is >85% pure by SDS-PAGE.
Recombinant HDAC6 (H230A) protein gel
HDAC6 (H230A) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
HDAC6 (H230A) Protein activity assay.
3 µM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (H230A) protein in reaction buffer for 30 minutes at 37°C followed by developing for 30 minutes at room temperature. Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.
HDAC6 (H230A) Protein activity assay.
3 µM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (H230A) protein in reaction buffer for 30 minutes at 37°C followed by developing for 30 minutes at room temperature. Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
