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Histone H4K20me3 antibody (mAb)
| RRID: AB_2650526 |
| Clone: 6F8-D9 |
| 商品コード: 39671 | Format: 100 µg | ¥92,000 | Buy |
| 商品コード: 39672 | Format: 10 µg | ¥25,000 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
CUT&RUN
ChIP
WB
ICC/IF
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H4 trimethyl Lys20 antibody (mAb) was raised against a peptide containing trimethyl Lys20 of Histone H4.
Buffer
Purified IgG in PBS with 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H4 trimethyl Lys20 mAb (Clone 6F8-D9) tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa chromatin (1.5 x 106 cell equivalents per ChIP) using 5 µg of Histone H4 trimethyl Lys20 antibody (mAb) (Clone 6F8-D9) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for human SAT-alpha repeats. Data are presented as Fold Enrichment of the ChIP antibody signal compared to the negative control IgG (which has been normalized to 1.0) using the ddCT method.
Histone H4K20me3 antibody (mAb) (Clone 6F8-D9) tested by Western blot.
HeLa nuclear extract (18 µg / lane) probed with Histone H4K20me3 antibody at 2 µg/ml.
Histone H4K20me3 antibody (mAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4K20me3 antibody (mAb) for trimethyl-Lys20 of histone H4. Peptides were spotted onto PVDF and probed with antibody at a dilution of 2 µg/ml. The amount of peptide (in picomoles) spotted is indicated (2, 10, and 50). Column 1: H4K20me0. Column 2: H4K20me1. Column 3: H4K20me2. Column 4: H4K20me3.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4K20me3 antibody (mAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
