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Histone H3S10ph antibody (mAb)
| RRID: AB_2793285 |
| Clone: MABI 0312 |
| 商品コード: 39636 | Format: 100 µg | ¥66,800 | Buy |
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Applications
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
IF
FC
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This Histone H3 phospho Ser10 antibody was raised against a peptide containing phospho serine 10 of human histone H3.
Buffer
PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
View our Guide to Histone Modifications and Biological Function.
Histone H3 phospho Ser10 mAb tested by immunofluorescence.
Staining of HeLa cells with Histone H3 phospho Ser10 mAb (1 µg/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H3S10ph antibody (mAb) tested by Western blot.
HeLa nuclear extract (30 µg per lane) was probed with Histone H3S10ph antibody at a 2 µg/ml dilution.
Lane 1: No treatment.
Lane 2: Cells treated with colcemid.
Histone H3 phospho Ser10 mAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: peptide phosphorylated at serine 10.
Lane 2: unmodified serine 10 peptide.
Lane 3: peptide phosphorylated at serine 28.
Lane 4: unmodified serine 28 peptide.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
This antibody is manufactured by MAB Institute, Inc.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
